Childhood Lymphoblastic Leukemia With Natural Killer Activity : Establishment of the Leukemia Cell Lines

Leukemic cells from a child with acute lymphoblastic leukemia (ALL) had high natural killer (NK) activity against K562 as determined by the 51Cr release assay at a 40:1 effector:target ratio: percent lysis was 76.8% (147.4% of normal lymphocyte value) and higher than that of control leukemic cells from 12 childhood ALL (0.1% ± 0.3%). Two leukemic cell lines (SPI-801 and SPI-802) were established from the patient, and they were essentially the same as the freshly harvested leukemic cells in their morphology. cytochemistry. immunologic markers. and functions. The cultured cell lines as well as the fresh leukemic cells had receptors for sheep red blood cells. IgG-Fc, and C3. The cultured cells were OKM1 . Ia and asialo-GM, and were OKT-3 . OKT-4 . OKT-6. OKT-8. Leu-7 . human mono-